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KMID : 1024420200240040327
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Food Engineering Progress
2020 Volume.24 No. 4 p.327 ~ p.335
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Evaluation of Stability and Antimicrobial Properties for a Bacteriocin from a Newly Isolated Lactobacillus gasseri G2
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Kim Jong-Hui
Lee Eun-Seon
Kim Bu-Min
Ham Jun-Sang
Oh Mi-Hwa
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Abstract
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Probiotic-derived bacteriocins have attracted considerable attention as an alternative to synthetic preservatives in the food industry, as synthetics are harmful to health. Lactobacillus gasseri G2 isolated from infant feces has been demonstrated to possess probiotic properties such as bile salt and acid tolerance, antibiotic resistance, and broad antimicrobial activity. Bacteriocin was partially purified by acetone precipitation and characterized for several properties.
It was found to be highly thermostable and resistant to heating at 120oC for 15 min. Bacteriocin activity remained unaffected with changes in the pH range from 2.0 to 10.0 and by solvents such as acetone, methanol, ethanol, and butanol. The antimicrobial activity was not affected by α-amylase, lysozyme, and lipase, but was reduced after treatment with protease, trypsin, and proteinase K, suggesting that it has a proteinaceous nature. Bacteriocin inhibited a broad range of target bacteria belonging to both Gram-positive and Gram-negative groups: Bacillus cereus, Listeria monocytogenes, Staphylococcus aureus, Clostridium perfringens, Escherichia coli O157:H7, Enterobacter sakasakii, Salmonella Enteritidis, Shigella flexneri, Shigella boydii, Vibrio parahaemolyticus, and Vibrio vulnificus. Bacteriocin appeared as a smear of the protein band with a molecular weight between 2-5 kDa, which showed a clear zone of inhibition after bioassay. The high thermostability and acid/alkali stability of bacteriocin reflect its potential commercial significance.
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KEYWORD
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Bacteriocin, Antimicrobial activity, Lactobacillus
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